Building a Cell from its Component Parts

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Download Building a Cell from its Component Parts written by Jennifer Ross, Wallace F. Marshall in PDF format. This book is under the category Biology and bearing the isbn/isbn13 number 012802450X/9780128024508. You may reffer the table below for additional details of the book.

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Specifications

book-author

Jennifer Ross, Wallace F. Marshall

publisher

Academic Press

file-type

PDF

pages

24 pages

language

English

asin

B010AS1XD4

isbn10

012802450X

isbn13

9780128024508


Book Description

The cell interior is a different world that we are only starting to explore. Though there are a number of approaches for examining the inner workings of the cell; the reductionist approach of building up complexity attracts many with physical science and engineering backgrounds. Building a Cell from its Component Parts; (PDF) spans a range of spatial scales from single protein molecules to vesicle and cell-sized structures capable of complicated behaviors. Contributions include; reconstituting membrane fission and fusion; methods to combine cytoskeletal assemblies with fabricated devices such as chambers or pillar arrays; methods for combining different motors and cytoskeletal components in defined ways to produce more complex behaviors; reconstituting important biological processes that normally take place on membrane surfaces; and procedures for encapsulating protein machines within vesicles or droplets.

NOTE: The product only includes the ebook; Building a Cell from its Component Parts in PDF. No access codes are included.

 

Additional information

book-author

Jennifer Ross, Wallace F. Marshall

publisher

Academic Press

file-type

PDF

pages

24 pages

language

English

asin

B010AS1XD4

isbn10

012802450X

isbn13

9780128024508

Table of contents


Table of contents :
Content: Front Cover
Methods in Cell Biology
Series Editors
Methods in Cell Biology
Copyright
Contents
Contributors
Preface
1. In vitro systems for the study of microtubule-based cell polarity in fission yeast
Introduction
1. Rationale
2. Materials
2.1 Materials for Method 1: Elongated Glass Wells with TiO2 Overhang
2.1.1 Microfabrication
2.1.2 Surface functionalization
2.1.3 Assay
2.2 Materials for Method 2: Elongated Water in Oil Emulsion Droplets
2.2.1 Microfluidic chip
3. Methods
3.1 Method 1: Elongated Glass Wells with TiO2 Overhang
3.1.1 Design 3.1.2 Deposition of chromium and titanium oxide by electron-beam evaporation3.1.3 Postannealing of titanium oxide
3.1.4 UV-lithography with S1813
3.1.5 Reactive ion etching
3.1.6 Wet etching with KOH
3.1.7 Surface functionalization
3.1.8 Assays
3.2 Method 2: Elongated Water in Oil Emulsion Droplets
3.2.1 Microfluidic design
3.2.2 Masks for microfluidic chip fabrication
3.2.3 SU-8 negative mould
3.2.4 PDMS chip (Figure 5)
3.2.5 Microfluidic setup and droplet formation
3.2.6 Lipids composition
3.2.7 Assays
Discussion and Perspectives
Acknowledgments
References 2. Microtubules, MAPs, and motor patternsIntroduction
1. Methods
2. Simple Filament-gliding Assay with Kinesin-1 and Microtubules
2.1 Flow Chambers
2.1.1 Materials
2.1.2 Chamber construction
2.1.3 Notes
2.2 Gliding Assay Reagents and Buffers
2.3 Experimental Details
2.4 Analysis and Notes
2.4.1 Kymographs
2.4.2 Filament end tracking
2.4.3 Notes
3. Gliding Assay with MAP65 Cross-linkers to Visualize Dynamic Cross-linking
3.1 MAP65 Purification
3.1.1 Preparation reagents and buffers
3.1.2 Protein purification
3.1.2.1 Day 1
3.1.2.2 Day 2
3.1.2.3 Day 3 3.2 Gliding Assay Buffers3.3 Experimental Details
3.4 Analysis and Notes
4. Cell-like Patterns from Gliding Prebundled Microtubule Filaments
4.1 Reagents and Buffers
4.2 Prebundling Microtubules
4.3 Experimental Details
Discussion and Summary
Acknowledgments
References
3. Self-organization of motors and microtubules in lipid-monolayered droplets
Introduction
1. Materials
1.1 Equipment
1.1.1 Glass surface treatment and Polydimethylsiloxane (PDMS) chamber preparation
1.1.2 Lipid-oil mixture preparation
1.1.3 Buffer droplet in oil production
1.1.4 Confocal fluorescence microscopy 1.2 Reagents1.2.1 For glass surface treatment and PDMS chamber preparation
1.2.2 For lipid-oil mixture preparation and buffer droplet in oil production
1.3 Buffers and Solutions
1.4 Proteins Used in the Assays
2. Methods
2.1 Glass Surface Treatment and PDMS Chamber Preparation
2.1.1 Cleaning and hydrophobic surface treatment
2.1.2 PDMS chambers
2.2 Lipid-Oil Mixture Preparation
2.3 Buffer Droplet in Oil Production
2.3.1 Droplet solution preparation
2.3.2 Resuspension of droplets in oil
2.4 Confocal Fluorescence Microscopy
Results and Discussion
Acknowledgments
References

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